Scalable production of high sensitive nanosensors based on graphene functionalized with a designed G-protein coupled receptor

그래핀 기반 FET소자는 높은 전하 이동도 및 Low level noise을 형성하여 Biosensor 로서의 높은 활용성이 기대된다. 본 논문에서 연구팀은 µ-opioid receptor protein을 결합시켜 그래핀 FET 소자 array 기반의 biosensor를 개발하고, 그 특성을 연구하였다. Shadow mask 기법을 사용하여 만들어진 그래핀 FET 소자 arry는 high quality and yield를 나타내었고, 이를 이용한 opioid biosensor는 high sensitivity and selectivity특성을 보여주었다.

Abstract : We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm2V−1s−1 and yield exceeding 98 %. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.

Authors : Mitchell B. Lerner, Felipe Matsunaga, Gang Hee Han, Sung Ju Hong, Jin Xi, Alexander Crook,
Jose Manuel Perez-Aguilar, Yung Woo Park, Jeffery G. Saven, Renyu Liu, and A. T. Charlie Johnson

Nano Lett., Article ASAP
DOI: 10.1021/nl5006349
Publication Date (Web): April 17, 2014