Physical principles underlying chromosome structure and epigenetic gene regulation
일시 : 2014-10-01 16:00 ~
연사 : 김하진 교수(울산과학기술대학)
담당 :
장소 : 56동106호
DNA sequence composition as a determinant of the chromosome structure?
The chromosome spends most of its lifetime in the interphase in which it is only loosely condensed and hence viewed as a dynamic polymer. Its structure and dynamics globally affect the gene expression but it is only recent that its average structure was presented through the Hi-C technique by showing the intra- and inter-chromosomal contact map of the whole DNA. We recently discovered that the Hi-C contact map highly and universally correlates with the local AT content. From all-atom molecular dynamics simulations, we hypothesized that the polycation-driven condensation of the DNA explains such global behavior. We proved the hypothesis by designing a simple in vitro system for the single molecule measurement of dsDNA-to-dsDNA interaction.
The chromosome spends most of its lifetime in the interphase in which it is only loosely condensed and hence viewed as a dynamic polymer. Its structure and dynamics globally affect the gene expression but it is only recent that its average structure was presented through the Hi-C technique by showing the intra- and inter-chromosomal contact map of the whole DNA. We recently discovered that the Hi-C contact map highly and universally correlates with the local AT content. From all-atom molecular dynamics simulations, we hypothesized that the polycation-driven condensation of the DNA explains such global behavior. We proved the hypothesis by designing a simple in vitro system for the single molecule measurement of dsDNA-to-dsDNA interaction.