Presentation Title:
Neuron-on-a-Chip: A novel research tool for basic neural and medical science

Yoonkey Nam
Dept. Bio and Brain Engineering, KAIST

Abstract
Advances in photolithography, surface chemistry, cell culture techniques, and
biosensors make it possible to consider the design of patterned neural networks in culture
dishes. This talk presents recent achievements that demonstrate that we can create micropatterns
of neurons that are alive and functional on top of planar microelectrode arrays
(MEAs). The bases for the advances include: (a) microcontact printing of proteins such
that they survive cell culture conditions for up to a month; (b) the faithful compliance of
the growing neurons to the underlying protein patterns; (c) microelectrode arrays suitable
for stimulation and recording of neurons; (d) advanced cell culture media capable of
supporting neurons in culture without use of serum containing media or other supporting
cells. The results shows that neurons can be confined to desired patterns for extended
periods of time and can be stimulated and recorded by MEAs. A few challenging topics
in basic neuroscience and medical science will be discussed in detail for potential applied
interdisciplinary research projects.